The answer to the "Question of the Day" from last week is that Louis Pasteur was did not have an appropriate solid growth medium he could use to properly isolate and bacteria in pure culture. He and other scientists at the time used things like potatoes or jelly/animal gelatin to grow bacteria on solid surfaces. However, this presented two problems:
1. Gelatin is liquid at 37o C. This temperature is consistent with body/blood temperature and ideal for growing bacteria that cause human diseases. Many of the scientists such as Pasteur and Koch, were attempting to isolate and show that specific bacteria caused specific human/mammalian diseases.
2. The bacteria could feed on gelatin and potatoes as a nutrient source (so they would eat away the solid surface or grow too quickly and not remain isolated).
It was not until Fannie Hesse, the housewife/assistant of Walter Hesse (one of Robert Koch's students) introduced her husband to agar-agar that this problem was solved. Agar is an extract from seaweed. Fannie used it in her kitchen as a solidifying agent to make jellies and pies. Agar remains solid at 37o C so there was no more headache of the solid media dissolving in the lab incubator before Walter could finish his experiments. In addition to the fact that it doesn't melt in the incubator, it had little nutritional value for the bacteria, so they don't eat it very quickly.
Walter was using his home lab at the time of this discovery, but he wrote a letter to his supervisor, Robert Koch. Koch then started using agar as well, and the rest is Microbiology history. Because of this new ingredient, Koch was able to isolate bacteria in pure culture on a solid medium. This is key to his development of The Germ Theory. Agar is now universally used as a solidifying agent in culture media and for other molecular biology techniques such as gel electrophoresis.
This article from 1938 honors Fannie Hesse's contribution the field of Bacteriology. As it states, " Her contribution to bacteriology makes her immortal."
